goat anti igf1r  (R&D Systems)

Journal: International Journal of Molecular Sciences

Article Title: Insulin-like Growth Factor 1, Growth Hormone, and Anti-Müllerian Hormone Receptors Are Differentially Expressed during GnRH Neuron Development

doi: 10.3390/ijms241713073

Figure Lengend Snippet: IGF1R is expressed in olfactory neurogenic niches at E12.5. ( A ) Coronal sections of E12.5 mouse embryo heads at VNO level were immunolabeled for IGF1R (green) and nTUBB3 (red) to reveal neuron cell bodies and axons. ( B ) High magnification of squared box area in ( A ). Solid arrowheads indicate examples of nTUBB3-positive cells/axons exiting the VNO that express IGF1R in the nasal parenchyma. White arrows indicate strong expression of IGF1R in neurogenic epithelia of the VNO and the OE. Single-channel images are shown beside each panel. Abbreviations: IFG1R, insulin-like growth factor 1 receptor; VNO, vomeronasal organ; OE, olfactory epithelium. Scale bars: 250 μm ( A ) and 50 μm ( B ).

Article Snippet: PA5-112901), goat anti-GHR (1:20; R&D Systems, Udine, Italy, cat. AF1360), goat anti-IGF1R (1:20; R&D Systems, cod.

Techniques: Immunolabeling, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Insulin-like Growth Factor 1, Growth Hormone, and Anti-Müllerian Hormone Receptors Are Differentially Expressed during GnRH Neuron Development

doi: 10.3390/ijms241713073

Figure Lengend Snippet: IGF1R and GHR are expressed by GnRH neurons migrating in the nasal parenchyma, but not by GnRH neurons residing in the VNO. ( A ) Coronal sections of E12.5 mouse embryo heads at the VNO level were immunolabeled for IGF1R (green) and GnRH (red) to reveal GnRH neurons. Solid arrowheads indicate examples of GnRH-positive cells exiting the VNO that express IGF1R. Empty arrowheads indicate examples of GnRH-positive cells within the VNO that do not express IGF1R. ( B ) Coronal sections of E12.5 mouse embryo heads at the VNO level were immunolabeled for GHR (green) and GnRH (red) to reveal GnRH neurons. Solid arrowheads indicate examples of GnRH-positive cells exiting the VNO that express GHR. Empty arrowheads indicate examples of GnRH-positive cells within the VNO that do not express GHR. Single-channel images are shown beside each panel. Abbreviations: IFG1R, insulin-like growth factor 1 receptor; GHR, growth hormone receptor; GnRH, gonadotropin-releasing hormone; VNO, vomeronasal organ. Scale bars: 50 μm.

Article Snippet: PA5-112901), goat anti-GHR (1:20; R&D Systems, Udine, Italy, cat. AF1360), goat anti-IGF1R (1:20; R&D Systems, cod.

Techniques: Immunolabeling

Journal: International Journal of Molecular Sciences

Article Title: Insulin-like Growth Factor 1, Growth Hormone, and Anti-Müllerian Hormone Receptors Are Differentially Expressed during GnRH Neuron Development

doi: 10.3390/ijms241713073

Figure Lengend Snippet: IGF1R is expressed by neurons and axons migrating/extending from the VNO. ( A , B ) Coronal sections of E14.5 mouse embryo heads at the VNO level were immunolabeled for IGF1R (green) and nTUBB3 (red) to reveal neuron cell bodies and axons. White arrows indicate the expression of IGF1R in the VNO/dorsal OE neurogenic epithelia. The high magnification of the squared box area is shown in ( B ). Solid arrowheads indicate examples of nTUBB3/IGF1R-positive cells/axons exiting the VNO. ( C , D ) Coronal sections of E14.5 mouse embryo heads at the OB level were immunolabeled for IGF1R (green) and nTUBB3 (red) to reveal neuron cell bodies and axons. The high magnification of the squared box area is shown in ( D ). Solid arrowheads indicate examples of nTUBB3/IGF1R-positive axons innervating the OB. Single-channel images are shown below each panel. Abbreviations: IFG1R, insulin-like growth factor 1 receptor; VNO, vomeronasal organ; OE, olfactory epithelium; OB, olfactory bulb; and NS, nasal septum. Scale bars: 250 μm ( A , C ) and 50 μm ( B , D ).

Article Snippet: PA5-112901), goat anti-GHR (1:20; R&D Systems, Udine, Italy, cat. AF1360), goat anti-IGF1R (1:20; R&D Systems, cod.

Techniques: Immunolabeling, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Insulin-like Growth Factor 1, Growth Hormone, and Anti-Müllerian Hormone Receptors Are Differentially Expressed during GnRH Neuron Development

doi: 10.3390/ijms241713073

Figure Lengend Snippet: IGF1R is not expressed by migrating and hypothalamic GnRH neurons. ( A , B ) Coronal sections of E14.5 mouse embryo heads at the VNO level were immunolabeled for IGF1R (green) and GnRH (red) to reveal GnRH neurons. The high magnification of the squared box area is shown in ( B ). Empty arrowheads indicate examples of GnRH-positive cells that are in close contact with IGF1R-positive axons but do not express IGF1R themselves. ( C , D ) Coronal sections of E14.5 mouse embryo heads at the CP level were immunolabeled for IGF1R (green) and GnRH (red) to reveal GnRH neurons. The high magnification of the squared box area is shown in ( D ). Empty arrowheads indicate examples of GnRH-positive cells that are in close contact with IGF1R-positive axons but do not express IGF1R themselves. ( E – H ) Coronal sections of E14.5 ( E , F ) and E18.5 ( G , H ) mouse embryo heads at the MPOA level were immunolabeled for IGF1R (green) and GnRH (red) to reveal GnRH neurons. The high magnification of the squared box area is shown in ( F ). Empty arrowheads indicate examples of GnRH-positive cells that do not express IGF1R. Note the high expression of IGFR in the OVLT area. Single-channel images are shown beside each panel. Abbreviations: VNO, vomeronasal organ; OE, olfactory epithelium; OB, olfactory bulb; and MPOA, medial preoptic area. Scale bars: 250 μm ( A , C , E , G ) and 50 μm ( B , D , F , H ).

Article Snippet: PA5-112901), goat anti-GHR (1:20; R&D Systems, Udine, Italy, cat. AF1360), goat anti-IGF1R (1:20; R&D Systems, cod.

Techniques: Immunolabeling, Expressing